CDT (carbohydrate deficient transferrin)-2 new use and method using CDT-2 to promote microbial cells to transport xylo-oligosaccharides and application thereof
The invention discloses a method for promoting microbial cells to transport and / or hydrolyze xylo oligosaccharides and application thereof. Experiments prove that cello-oligosaccharide transport protein CDT-2 also has xylo-oligosaccharide transport capacity, beta xylosidase GH43-2 has hydrolysis e...
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Main Authors | , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
12.11.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a method for promoting microbial cells to transport and / or hydrolyze xylo oligosaccharides and application thereof. Experiments prove that cello-oligosaccharide transport protein CDT-2 also has xylo-oligosaccharide transport capacity, beta xylosidase GH43-2 has hydrolysis effect on the xylo-oligosaccharides, according to the method, cdt-2 gene and / or gh43-2 gene are/ is introduced into a microbial strain to obtain a recombinant microbial strain, and compared with a starting strain, the recombinant microbial strain acquires or improves the capability for transporting the xylo-oligosaccharides from extracellular to intracellular and / or hydrolyzing the xylo-oligosaccharides into xylose. By discovery of the xylo-oligosaccharide transport protein CDT-2 and application of the beta xylosidase GH43-2, an idea using modified yeast to directly use the xylo-oligosaccharides derived from hemicellulose degradation for producing biological chemicals is provided, co-fermentation of glucose, xyl |
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Bibliography: | Application Number: CN201310165380 |