Continuous flow method for measuring total alkaloid in tobacco or tobacco products

• Main Authors: HOU HONGWEI, FENG XIAOMIN, LIU NAN, LUO ANNA, HE SHENGBAO, ZHANG WEI, XING JUN, MA YANJUN, HU QINGYUAN, WANG YINGYUAN
• Format: Patent
• Language: Chinese; English
• Published: 05.11.2014
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention provides a continuous flow method for measuring total alkaloid in tobacco or tobacco products. In the method, potassium thiocyanate and sodium dichloro isocyanurate are adopted to carry out reactions to generate cyanogen chloride on line. The cyanogen chloride can carry out reactions with total alkaloid (calculated in the form of nicotine) in tobacco or tobacco products to break off the pyridine rings of nicotine, and then further carries out reactions with p-aminobenzene sulfonic acid. The reaction products are measured by a chromometer at 460 nm. The method uses a buffer system to control the pH value of the reaction system in a range of 6.0 to 7.5, and the buffer system is composed of a buffer solution A and a buffer solution B. The buffer solution A is prepared by the following steps: weighing disodium hydrogen phosphate and trisodium phosphate, placing the weighed substances in a beaker, dissolving the substances with water, transferring the solution to a volumetric flask (1L), and adding w