Method for cultivating liquid cultures of cordyceps militaris
The invention discloses a method for cultivating liquid cultures of cordyceps militaris. The method includes the steps that firstly, a culture medium is prepared; secondly, bottling and sterilization are carried out, specifically, the prepared culture medium is put into a culture bottle, and the cul...
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Main Authors | , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
18.06.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a method for cultivating liquid cultures of cordyceps militaris. The method includes the steps that firstly, a culture medium is prepared; secondly, bottling and sterilization are carried out, specifically, the prepared culture medium is put into a culture bottle, and the culture bottle filled with the prepared culture medium is placed into an autoclave to be sterilized after being sealed through a sealing film; thirdly, inoculation is carried out, and specifically the culture solution is inoculated with slant stock cultures of the cordyceps militaris after sterilization is completed and the culture solution is cooled; fourthly, cultivation is carried out, specifically, the culture bottle filled with the culture solution inoculated with the slant stock cultures is placed into an incubator, cultivation is carried out in a light-proof and airtight environment, and the liquid cultures of the cordyceps militaris can be obtained after 6-8 days of cultivation. According to the method, device |
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Bibliography: | Application Number: CN20141110255 |