Application of directionally mutated galactosyl transferase

• Main Authors: CHEN HUAN, JOSEF VOGLMEIR, LIU LI
• Format: Patent
• Language: Chinese; English
• Published: 11.06.2014
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CARE OF BIRDS, FISHES, INSECTS; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; DAIRY PRODUCTS, e.g. MILK, BUTTER, CHEESE; ENZYMOLOGY; FISHING; FOODS OR FOODSTUFFS; FORESTRY; HUMAN NECESSITIES; HUNTING; MAKING THEREOF; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MILK OR CHEESE SUBSTITUTES; MUTATION OR GENETIC ENGINEERING; NEW BREEDS OF ANIMALS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; SPIRITS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; TRAPPING; VINEGAR; WINE

The invention discloses an application of directionally mutated galactosyl transferase. The research shows that the directionally mutated galactosyl transferase 1 can be changed to only transfer acetyl galactosamine from transferring wild type galactose, and unlike the prior art that the mutated beta1,4-galactosyl transferase 1 can simultaneously transfer galactose and acetyl galactosamine, and the transferring efficiencies of the directionally mutated galactosyl transferase and the beta1,4-galactosyl transferase 1 are both approximate to 50%, the directionally mutated galactosyl transferase 1 and the beta1,4-galactosyl transferase 1 are greatly different from each other. Therefore, the directionally mutated galactosyl transferase 1 can be applied to production of natural milk without lactose or with very low lactose content. The mutated galactosyl transferase 1 can be changed to transfer acetyl galactosamine from transferring galactose, so that the lactose components in milk are changed and the milk is conve