Separating and purifying method of angiotensin converting enzyme inhibitor in ramulus mori skin
The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus...
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Main Authors | , , |
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Format | Patent |
Language | Chinese English |
Published |
04.06.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus mori skin fluid suspension, adding cellulase 3200-3800U/g, adjusting pH value to 4.5-5.0, carrying out supersonic wave cell wall breaking extraction, separation and filtering, and isolating supernatant to obtain ramulus mori skin leach liquid; 3, isolating anion resin 201X7 exchange column chromatography and sephadex G-25 from the ramulus mori skin leach liquid, and freeze drying the isolated products so as to obtain the angiotensin converting enzyme inhibitor in single component and high output; the method is simple in technology, and easy in industrialization scale production. |
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Bibliography: | Application Number: CN2014155650 |