Separating and purifying method of angiotensin converting enzyme inhibitor in ramulus mori skin

The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus...

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Bibliographic Details
Main Authors JIA JUNQIANG, LIU YANWEI, GUI ZHONGZHENG
Format Patent
LanguageChinese
English
Published 04.06.2014
Subjects
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
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Summary:The invention discloses a separating and purifying method of an angiotensin converting enzyme inhibitor in ramulus mori skin; the method comprises steps of: 1, peeling off and crushing and processing the ramulus mori skin; 2, weighting crushed and screened ramulus mori skin, adding water and ramulus mori skin fluid suspension, adding cellulase 3200-3800U/g, adjusting pH value to 4.5-5.0, carrying out supersonic wave cell wall breaking extraction, separation and filtering, and isolating supernatant to obtain ramulus mori skin leach liquid; 3, isolating anion resin 201X7 exchange column chromatography and sephadex G-25 from the ramulus mori skin leach liquid, and freeze drying the isolated products so as to obtain the angiotensin converting enzyme inhibitor in single component and high output; the method is simple in technology, and easy in industrialization scale production.
Bibliography:Application Number: CN2014155650