Method for detecting viruses and bacteria by fluorescent targeted cells

• Main Authors: PANG DAIWEN, CUI RAN, XIONG LINGHONG
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2014
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method which is capable of efficiently converting a cell into a biological probe and applying the biological probe into the detection for virus and bacteria. The method comprises the steps of successfully applying the original method with 'space-time coupling' new strategy put forward by the laboratory into staphylococcus aureus to enable the interior of the bacteria to be lightened by luminescent quantum dots, and ingeniously establishing a signal report body; due to the natural expression protein A on the surfaces of the cells, carrying out incubation on the fluorescent bacteria and antibody without complex metabolism and genetic engineering cell modification, and carrying out centrifugal washing on excessive antibody to enable the surface of cell wall to be combined with the antibody to obtain the probe with targeting property. The method can be used for capturing the virus or bacteria to be detected by an immune magnetic ball by being combined with an immune magnetic trapping me