Immune colloidal gold rapid detection test strip for staphylococcus aureus enterotoxin E and preparation method thereof

• Main Authors: KUANG HUA, KONG DEZHAO, XU LIGUANG, XU CHUANLAI, SONG SHANSHAN, LIU LIQIANG, MA WEI
• Format: Patent
• Language: Chinese; English
• Published: 07.05.2014
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to an immune colloidal gold rapid detection test strip for staphylococcus aureus enterotoxin E and a preparation method of the immune colloidal gold rapid detection test strip, and belongs to the technical field of immune detection. The test strip is composed of a sample cushion, a gold label cushion coated with a gold label marked anti-SEE antibody 1E5 (CGMCC (China General Microbiological Culture Collection Center) No.7214), and a nitrocellulose membrane which is respectively coated with an anti-SEE antibody 2E3 (CGMCC No.7215) and goat anti-mouse IgG on a detection line and a quality control line; the sample cushion, the gold label cushion, the nitrocellulose membrane and a water absorption cushion are sequentially stuck on a PVC (Polyvinyl Chloride) bottom plate. The test strip is simple and convenient, rapid and accurate to operate; a whole testing process only needs 5 minutes and is not interfered by environment conditions; the immune colloidal gold rapid detection test strip has g