Kit and method of detecting the resistant microorganisms to a therapeutic agent

• Main Authors: SANTOS SILVA DE FARIA RAMOS ANTUNES ISABEL CRISTIN, PINTO E SILVA ANA TERESA, DOS SANTOS ROCHA DO ROSARIO RITA MAFALDA, QUINTA E COSTA DE OLIVEIRA MORAIS ANA SOFIA, AGOSTINHO GONCALVES RODRIGUES ACACIO, AZEVEDO PINA VAZ CIDALIA IRENE
• Format: Patent
• Language: Chinese; English
• Published: 09.04.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The present invention relates to a method for detecting resistant microorganisms to a therapeutic agent in a biological sample, comprising the following steps: a. inoculate the said sample, uncultured, on a first tube with, and on a second tube without, at least one therapeutic agent; preferentially further including at least one lysing agent and/or a buffer, and/or a suitable culture medium, or put the sample on a separation serum tube; and incubated it; b. add to both tubes a fluorescent marker; c. perform a fluorescence analysis for obtaining one or more fluorescence or growth parameters for each of the two tubes; wherein the microorganisms resistant phenotype of the biological sample to said therapeutic agent is obtained by comparing the one or more fluorescence parameters between the two tubes. Therefore, the present invention is useful in laboratory procedures or routines for the detection of the susceptibility of different microorganisms to a therapeutic agent, to the determination of microorganisms re