Saccharifying enzyme and engineering strain for realizing recombinant expression of same

The invention provides a saccharifying enzyme of which the amino acid sequence is SEQ ID NO:1. The optimum operative temperature of the saccharifying enzyme is 75 DEG C, and 80% or above of the enzyme activity can be kept in a range of 70-80 DEG C; and the optimum operative pH value is 6.0, and 70%...

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Bibliographic Details
Main Authors WU JIAPENG, ZHANG HUIDAN, HUANG YIJUN, LIU SHICHENG, ZHU QIANQIAN
Format Patent
LanguageEnglish
Published 17.06.2015
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a saccharifying enzyme of which the amino acid sequence is SEQ ID NO:1. The optimum operative temperature of the saccharifying enzyme is 75 DEG C, and 80% or above of the enzyme activity can be kept in a range of 70-80 DEG C; and the optimum operative pH value is 6.0, and 70% or above of the enzyme activity can be kept in a pH value range of 4.0-7.0. The saccharifying enzyme can be widely used in production of glucose, and the DE value (reducing sugar value) is up to a maximum of 97% after 2.5h; and the saccharifying enzyme is high in specificity and can efficiently decompose alpha-1,4-glycosidic bonds, and the glucose content in the product is up to 90%. Besides, the saccharifying enzyme has favorable temperature tolerance, so that the saccharifying enzyme can be directly added to perform enzymolysis reaction without adding a cooling step after starch liquefaction, thereby saving work time and energy, saving more production cost and having wide market prospects.
Bibliography:Application Number: CN201310719904