Method for high-yield preparation of glycyrrhetinic acid monoglucuronide

• Main Authors: YI MING, HUANG QUANSHU, YUAN JIWEN, YANG YONGAN, JI HAO, WEI YUANGANG, ZHONG HUI, JIN XIANYOU
• Format: Patent
• Language: Chinese; English
• Published: 15.01.2014
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; SPIRITS; STEROIDS; SUGARS; VINEGAR; WINE

The invention provides a method for high-yield preparation of glycyrrhetinic acid monoglucuronide, and belongs to the technical field of biochemistry. The method comprises the following steps: with a bacterial strain of generating specific glucuronidase as a catalyst and glycyrrhizic acid as a substrate, removing a glycyrrhetinic acid monoglucuronide product by taking a macroporous resin as a separating medium in processes of continuous stirring and ventilating conversion reaction, releasing the substrate glycyrrhizic acid adsorbed in the resin, and discharging the glycyrrhizic acid out of a resin column together with unreacted glycyrrhizic acid in a fermentation liquor; and reflowing to a fermentation tank to continue to ferment. Simultaneous coupling of three processes of fermentation, material supplement and product separation is achieved; the problems of negative feedback inhibition and unfavourable fermentation caused by over-high viscosity of the product and a substrate fermented liquid are solved by ma