Novel alkali-resistant variants of protein a and their use in affinity chromatography

The present invention relates to immunoglobulin (Ig)-binding proteins with alkali- resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a...

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Bibliographic Details
Main Authors TAMORI KOUJI, LI XING, SHIOTANI TOMONORI, FUKUTA TETSUO, NAKAMURA SATOSHI, LI SHUNNG, OKANO YUUSUKE, VOSS COURTNEY
Format Patent
LanguageChinese
English
Published 20.11.2013
Subjects
CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
PERFORMING OPERATIONS
PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
SEPARATION
THEIR RELEVANT APPARATUS
TRANSPORTING
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Summary:The present invention relates to immunoglobulin (Ig)-binding proteins with alkali- resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.
Bibliography:Application Number: CN2011861195