Stenotrophomonassp. H-4 and preparation method of degrading enzyme preparation thereof as well as application

• Main Authors: MA WENTING, CHEN YONG, LI ZHENGAO, CHEN TING, JIANG RUI, ZHAO QIGUO, JIANG ZHIWEI, TENG YING, ZHANG MANYUN, LUO YONGMING
• Format: Patent
• Language: Chinese; English
• Published: 18.09.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FOODS OR FOODSTUFFS; FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J; HUMAN NECESSITIES; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES; VINEGAR; WINE

The invention provides stenotrophomonassp. H-4 and a preparation method of degrading enzyme preparation thereof as well as application. The stenotrophomonassp. H-4 has been conserved in the conservation unit specified by the State Intellectual Property Office; the conservation date is February 1, 2013; the name of the conservation unit is China General Microbiological Culture Collection Center; and the conservation number is CGMCC No.7274. The stenotrophomonassp. H-4 can be grown on a culture medium with chlorothalonil as the unique carbon source and energy, and after being cultivated for 7 days, the degradation rate of the stenotrophomonassp. H-4 reaches 83.6%; the enzyme activity is 3.28 U and the degradation rate of the degrading enzyme to the chlorothalonil is 86% when the extraction conditions for crude enzyme in the stenotrophomonassp. H-4 are as follows: ultrasonic powder: 30 W; and ultrasonic time: 5 minutes. Obviously, the degrading enzyme preparation provided by the invention has bright application