Recombinant factor C from Tachypleus tridentatus expressed by insect cells

The invention relates to the field of bioengineering, in particular to a method for recombining and expressing a gene of a factor C from Tachypleus tridentatus in insect cells so as to detect and remove endotoxin. The method comprises the steps of preparing a recombinant rhabdovirus of the gene of t...

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Bibliographic Details
Main Authors ZHUANG JIALIN, GUO HENGCHANG, MOU ZONGCHUN, GAO XUECHAO, ZHOU KANG
Format Patent
LanguageChinese
English
Published 11.09.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention relates to the field of bioengineering, in particular to a method for recombining and expressing a gene of a factor C from Tachypleus tridentatus in insect cells so as to detect and remove endotoxin. The method comprises the steps of preparing a recombinant rhabdovirus of the gene of the factor C from Tachypleus tridentatus, transfecting the insect cells, detecting the expression of the recombinant factor C from Tachypleus tridentatus in the insect cells, and the like. The recombinant factor C from Tachypleus tridentatus, prepared by the method, provides a precondition for researching the binding activity of endotoxin of the factor C, and also lays a foundation for the application of the factor C in detection and removal of the endotoxin.
Bibliography:Application Number: CN2012146099