Method for quickly screening antibiotics-producing actinomycetes from soil

The invention discloses a method for quickly screening antibiotics-producing actinomycetes from soil. The method comprises the following steps of: acquiring a soil sample; culturing, wherein the sample series are inoculated to a culture dish containing Gause culture medium No.1 and cultured for 6-12...

Full description

Saved in:
Bibliographic Details
Main Authors FU GUIRONG, GUO QIANQIAN, CHEN GUOHUA
Format Patent
LanguageChinese
English
Published 04.09.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention discloses a method for quickly screening antibiotics-producing actinomycetes from soil. The method comprises the following steps of: acquiring a soil sample; culturing, wherein the sample series are inoculated to a culture dish containing Gause culture medium No.1 and cultured for 6-12 days after being diluted in a gradient manner; picking out single colonies, wherein all single colonies in the culture dish with the most single colonies are picked out and inoculated; inoculating in a rectangular manner, wherein all single colonies are numbered and inoculated onto a new Gause culture medium No.1; drawing short lines and inoculating to the new culture medium; screening: punching the culture medium for growing actinomycetes into circular agar blocks by using a stainless steel puncher, and picking up the agar blocks by tweezers, placing onto the culture medium which is -coated with test bacteria in advance and culturing for 18-24 hours; and analyzing the antibacterial activity by adopting a transpar
Bibliography:Application Number: CN20131202954