Production method of raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry

• Main Authors: HUO NAN, GAO XUEJUN, YUAN XIAOHAN, YU WEI, ZHANG MINGHUI, HAO JINGYOU, LIU YING, QIU YOUWEN, LI QINGZHANG
• Format: Patent
• Language: Chinese; English
• Published: 21.08.2013
• Subjects: FODDER; FOODS OR FOODSTUFFS; HUMAN NECESSITIES; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES

The invention discloses a production method of a raw milk lactic acid bacteria antimicrobial peptide additive for livestock and poultry, relates to a production method of fermentation in various plant feed proteins and solves problems that the production strains of the antimicrobial peptide additive for the livestock and the poultry is undefined and the activity of antimicrobial peptide is low. The production method comprises the following steps that 1, frozen Lactobacillus acidophilus strains and Lactobacillus casei are activated by MRS culture mediums and then are respectively inoculated in enrichment mediums so that the enrichment culture solution is obtained; 2, the enrichment culture solution is inoculated in a seed culture medium and then is fermented at 35-40 DEG C to obtain a seed culture solution; 3, the seed culture solution is inoculated to the fermentation culture medium again to be fermented to obtain fermentation products; and 4, the fermentation products are dried, dehydrated, crushed and mixed