Protein isoelectric focusing method and apparatus

The invention provides a protein isoelectric focusing method based on moving reaction boundary and diffusion technology and an apparatus thereof. According to the invention, a porous hydrophilic material containing electrode liquid is prepared at first and is used in an acidic pad and an alkaline pa...

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Bibliographic Details
Main Authors LI SI, CAO CHENGXI, LIU XIAOPING, WANG HOUYU, GUO CHENGANG
Format Patent
LanguageChinese
English
Published 07.08.2013
Subjects
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Summary:The invention provides a protein isoelectric focusing method based on moving reaction boundary and diffusion technology and an apparatus thereof. According to the invention, a porous hydrophilic material containing electrode liquid is prepared at first and is used in an acidic pad and an alkaline pad; then the acidic pad is disposed between an anode in an electrophoresis tank and one end of a mobilized or immobilized pH gradient strip which has undergone hydration and sample loading; the alkaline pad is disposed between a cathode and the other end of the strip and is slightly pressed and fixed so as to realize electric connection; and finally, silicone oil is added drop by drop to cover the whole interior of the electrophoresis tank, a direct voltage is applied, and the voltage is gradually increased so as to realize isoelectric focusing test. The objective of the invention is to improve adjustability, controllability and distinguishability of a pH gradient.
Bibliography:Application Number: CN20131113855