Plum blossom genetic map construction method

• Main Authors: ZHANG JIE, ZHANG QIXIANG, XU ZONGDA, CHEN JINGXIN, SUN LIDAN
• Format: Patent
• Language: Chinese; English
• Published: 03.07.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention provides a plum blossom genetic map construction method comprising the steps that: (1) a plum blossom F1 generation testcross population is constructed; (2) parents and offspring genome DNA thereof are extracted; (3) with a DNA sequencing method related to restriction endonuclease loci, the parents and offspring genome are re-sequenced, such that a single-nucleotide polymorphism marker satisfying the Mendelian inheritance law is obtained; and (4) according to the obtained single-nucleotide polymorphism marker, the plum blossom genetic map is constructed by using a testcross strategy. According to the invention, plum blossom variety hybridization problems of low seed setting rate, long breeding cycle, and difficulty in constructing F1 population are overcome, and the plum blossom genetic map is rapidly constructed with an RAD and testcross strategy combined method.