Plum blossom genetic map construction method

The invention provides a plum blossom genetic map construction method comprising the steps that: (1) a plum blossom F1 generation testcross population is constructed; (2) parents and offspring genome DNA thereof are extracted; (3) with a DNA sequencing method related to restriction endonuclease loci...

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Bibliographic Details
Main Authors ZHANG JIE, ZHANG QIXIANG, XU ZONGDA, CHEN JINGXIN, SUN LIDAN
Format Patent
LanguageChinese
English
Published 03.07.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a plum blossom genetic map construction method comprising the steps that: (1) a plum blossom F1 generation testcross population is constructed; (2) parents and offspring genome DNA thereof are extracted; (3) with a DNA sequencing method related to restriction endonuclease loci, the parents and offspring genome are re-sequenced, such that a single-nucleotide polymorphism marker satisfying the Mendelian inheritance law is obtained; and (4) according to the obtained single-nucleotide polymorphism marker, the plum blossom genetic map is constructed by using a testcross strategy. According to the invention, plum blossom variety hybridization problems of low seed setting rate, long breeding cycle, and difficulty in constructing F1 population are overcome, and the plum blossom genetic map is rapidly constructed with an RAD and testcross strategy combined method.
Bibliography:Application Number: CN20111454348