Aptamer capable of simultaneously identifying OdDHL ([N-(3-oxododecanoyl)-L-homoserine lactone]) and BHL (N-butanoyl-L-homoserine lactone) and application thereof

• Main Authors: ZHAO ZUGUO, YANG ZAIMING, WU SHUQING
• Format: Patent
• Language: Chinese; English
• Published: 26.06.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS; SPIRITS; VINEGAR; WINE

The invention relates to the field of biotechnology, and in particular relates to an aptamer capable of simultaneously identifying OdDHL ([N-(3-oxododecanoyl)-L-homoserine lactone]) and BHL (N-butanoyl-L-homoserine lactone) and an application thereof. OdDHL and BHL are signaling molecules of las and rhl signaling pathways of a pseudomonas aeruginosa population effect system, respectively; and the activation of the two signaling pathways is capable of starting expression of a pseudomonas aeruginosa virulence gene. An aptamer bonding OdDHL and BHL in high affinity is screened by using a competition method. The aptamer has a population effect system for interfering pseudomonas aeruginosa and the potential for inhibiting the expression of the pseudomonas aeruginosa virulence gene. The affinity of bonding the aptamer with OdDHL and BHL is equivalent to that of an antibody, and the preparation process is simple and easy to operate and low in cost.