Method for determining cancer onset or cancer onset risk

• Main Authors: GONDA KOHSUKE, TAKEDA MOTOHIRO, OHUCHI NORIAKI, MIYASHITA MINORU
• Format: Patent
• Language: Chinese; English
• Published: 19.06.2013
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

Provided is a method for highly accurately and quantitatively determining cancer onset or cancer onset risk by quantitative tissue-staining which is conducted in a biotissue with the use of an antibody capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, for example, PAR1 antibody inhibiting the mobility and infiltrating properties of cancer cells. Cancer onset or cancer onset risk is determined by a tissue staining method, said method comprising: a step for labeling an antibody, which is capable of recognizing a cancer proliferation inhibitor or a cancer metastasis inhibitor, with a fluorescent substance and then contacting the fluorescent-labeled antibody with a tissue sample; a step for irradiating a tissue site, which has been contacted with said antibody, with an excitation light to give a fluorescence image; a step for acquiring an intrinsic fluorescence image in a neighborhood region on a shorter or longer wavelength side, compared with the region wherein the wavele