Complex enzyme preparation for leaching tobacco material and enzyme leaching method

• Main Authors: ZHANG LITAO, WANG WENLING, SHANG FENG, WANG HONGTAO, XU HONGTAO, SHANG FUDE
• Format: Patent
• Language: Chinese; English
• Published: 12.06.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CIGARETTES; CIGARS; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING ORCHEWING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SMOKERS' REQUISITES; SNUFF; SPIRITS; TOBACCO; VINEGAR; WINE

The invention relates to a complex enzyme preparation for a leaching tobacco material and an enzyme leaching method, and belongs to the technical field of regenerated tobacco processing. The active component of the complex enzyme preparation comprises 0.1-1000U of lysozyme, 1-1000 U of cellulase, 0.1-1000U of alpha-amylase, 0.1-1000 U of proteolytic enzyme and 0.1-1000U of pectinase, wherein the amount is the adding amount in per gram of tobacco material. The temperature in leaching is controlled at 30-60 DEG C, and the liquor ratio is 1:5-10; the enzyme preparation is added to be stirred for 1-4 hours; and after solid-liquid separation, solid phase is taken and water is further added according to original solid weight for circularly extraction for 1-2 times. According to the invention, mesophyll cell and cabo fiber structures, composition, physical properties and the like of tobacco are adequately considered, combined wall breaking effects of lysozyme, pectinase and cellulase are introduced, and enzyme catal