Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

• Main Authors: TRIPATHI SHITAL A, CHIU YIN-YING, DELEAULT KRISTEN M, DEN HAAN RIAAN, BELCHER ALAN, HAU HEIDI H, MELLON MARK, RAJGARHIA VINEET B, XU HAOWEN, SHIKHARE INDRANEEL, RICE CHARLES F, GOSSELIN JENNIFER, WENGER KEVIN S, SKINNER RYAN, ARGYROS AARON, WARNER ANNE K, GANDHI CHHAYAL V, MCBRIDE JOHN E, CAIAZZA NICKY, BARRETT TRISHA, WISWALL ERIN, STONEHOUSE EMILY, FROEHLICH ALLAN C, AGBOGBO FRANK, BREVNOVA ELENA, FOSTER ABIGAIL S, BARDSLEY JOHN S
• Format: Patent
• Language: Chinese; English
• Published: 29.05.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that, full suite, of enzymes to hydrolyze com starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicelhiiose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction.; The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase