5'terminal flanking region and 3'terminal untranslated region of mutational A2M (Alpha 2 Macroglobulin) gene, as well as detection primer, kit and detection method thereof

• Main Authors: ZHONG JIFENG, HUANG JINMING, YUAN JINDUO, WANG XIUGE, JU ZHIHUA
• Format: Patent
• Language: Chinese; English
• Published: 15.05.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a 5'terminal flanking region of a mutational A2M (Alpha 2 Macroglobulin) gene, and the sequence of the 5'terminal flanking region is shown as SEQ ID NO.8. The invention discloses a 3'terminal untranslated region of the mutational A2M gene, and the sequence of the 5'terminal flanking region is shown as SEQ ID NO.7. The experiment shows that the 5'terminal flanking region of the mutational A2M gene, which is used as a promoter, has a stronger promoting activity; the 3'terminal untranslated region of the mutational A2M gene can influence the bonding capability of mi-2898; and the bonding capability of the mutated 3'terminal untranslated region and the mi-2898 is weaker than that of the non-mutated 3'terminal untranslated region and the mi-2898, so that the two types of mutations can be used as functional molecular markers of a candidate gene for the breeding improvement process of dairy cow so as to perform an assistant selection of individual cow with higher mastitis resistance. Thus, ba