Y-probe and variation thereof, and DNA microarray, kit, and gene analysis method using the Y-probe and the variation thereof

• Main Authors: OH MYUNG-RYURL, MOON WOOUL
• Format: Patent
• Language: Chinese; English
• Published: 08.05.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; DERIVATIVES THEREOF; ENZYMOLOGY; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PHYSICS; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; SUGARS; TESTING; VINEGAR; WINE

The present invention relates to a Y-type nucleotide probe having two probe portions on a single body such that it can be widely used for genotype testing and analysis with improved sensitivity, specificity, and accuracy, as well as to a DNA microarray, kit, and gene analysis method using the y-probe. The Y-probe of the invention is configured with five portions, comprising: a left probe portion, a left stem portion, a linker, a right stem portion, and a right probe portion. The DNA microarray of the present invention may have improved testing accuracy as it can perform a simultaneously overlapping test on the same gene or a simultaneous test on two different target genes. In particular, by simultaneously testing a target gene and a control gene in one spot, analysis errors can be reduced, quantitative analysis is made possible, and standardization is facilitated. The Y-probe of the present invention can be used for genotype analysis and gene expression analysis, as well as for mutation and SNP analysis, and