Mutant phage E gene, punching plasmid vector containing the gene and application thereof in preparation of vaccine

The present invention relates to the field of genetic engineering, and discloses DNA of PhiX174 phage E gene obtained after gene mutation. The serial number of the DNA is shown as SEQ ID NO:1. The invention also discloses a preparation method of the DNA, a punching plasmid vector containing the DNA...

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Bibliographic Details
Main Authors CHEN RUIAI, ZHANG DONGXIA, TANG QIN, XU JIAHUA, HUANG MIAORONG
Format Patent
LanguageChinese
English
Published 17.04.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:The present invention relates to the field of genetic engineering, and discloses DNA of PhiX174 phage E gene obtained after gene mutation. The serial number of the DNA is shown as SEQ ID NO:1. The invention also discloses a preparation method of the DNA, a punching plasmid vector containing the DNA and application thereof in the preparation of a vaccine. The expression quantity of mutant phage E gene of the present invention in poultry salmonella is much higher than that of conventional phage E gene in poultry salmonella. Compared with the conventional phage E gene, cleavage rate of the mutant phage E gene of the present invention for poultry salmonella is improved by 30-70%. The possibility of bacterial ghost of the poultry salmonella as a candidate vaccine is increased.
Bibliography:Application Number: CN20121541108