Method for producing cordycepin through semi-continuous liquid fermentation of cordyceps militaris

• Main Authors: WEI ZHAOJUN, ZHANG JIANGUO, FANG TINGTING
• Format: Patent
• Language: Chinese; English
• Published: 13.03.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION ORPROCESSING OF GOODS; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS; TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINSTCLIMATE CHANGE; VINEGAR; WINE

The invention discloses a method for producing cordycepin through semi-continuous liquid fermentation of cordyceps militaris, and the method comprises the following concrete operating steps of: (1) putting hyphae of the Cordyceps militaris on a PDA (Potato Dextrose Agar) solid culture medium and activating to generate a bacterial lawn with a plenty of dense mycelia; (2) putting the bacterial lawn on a seed culture medium, carrying out shake cultivation, and forming a plenty of mycelium pellets after 5 days, thereby obtaining seed liquid; (3) transferring the seed liquid into a seed liquid fermentation tank to carry out expanding culture; (4) transferring the seed liquid after being subjected into the expanding culture in a 500L tank to carry out first-batch fermentation; and (5) carrying out semi-continuous fermentation in 10 batches in the 500L tank under the same conditions. In the fermentation process, a method of adjusting the culture medium composition and the fermentation conditions is adopted to contro