Recombinant plasmid containing VEGFA (Vascular Endothelial Growth Factor) gene 3'UTR (Untranslated Region) and reporter gene

The invention discloses a recombinant plasmid containing a VEGFR (Vascular Endothelial Growth Factor) gene 3'UTR (Untranslated Region) and a reporter gene, wherein the reporter gene is a firefly luciferase gene. A preparation method of the recombinant plasmid comprises the steps of: 1, amplifyi...

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Main Authors JIN BAOHUI, WANG YIFEI, YIN PEIHAO, ZHOU LIHONG, HOU LILI, LI QI, HU SONGJIAO, ZHENG YUANHONG, LIU XUAN, YE NAIJING, LIANG BO, QIU YANYAN, QIN JIANMIN, CHEN XINGZHU, WANG YAN
Format Patent
LanguageChinese
English
Published 30.01.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Summary:The invention discloses a recombinant plasmid containing a VEGFR (Vascular Endothelial Growth Factor) gene 3'UTR (Untranslated Region) and a reporter gene, wherein the reporter gene is a firefly luciferase gene. A preparation method of the recombinant plasmid comprises the steps of: 1, amplifying and extracting a carrier plasmid; 2, cloning, digesting and purifying a 3'UTR sequence of the VEGFR gene; 3, connecting the 3'UTR sequence of the VEGFR gene with the carrier plasmid; and 4, screening a recombinant. The recombinant containing the VEGFR gene and the 3'UTR reporter gene, provided by the invention, has excellent application prospect on the aspects of screening angiogenesis related microRNA of, researching an action mechanism of angiogenesis and the microRNA, and researching the mechanism of tumor cell angiogenesis.
Bibliography:Application Number: CN20121461114