Monocotyledon transgenic method for invading growing points of seed buds minimally and fully

• Main Authors: SHI XUEPING, CHAI JIANFANG, ZHU JINYONG, ZHAO HE, YANG FAN, REN ZHIHENG, ZHANG QIANG, GAO YIPING, MA HUIJIE, ZUO WENBO, SUN GUOZHONG, WANG HAIBO, WANG ZHANWU, LIANG XINCHAO, XU XIAN, GUAN JUNFENG, ZHANG YANMIN, LV MENGYU, LIU YONGWEI, DONG FUSHUANG, HAN QIUFEN, ZHANG HUANHUAN
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2013
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to a monocotyledon transgenic method for invading growing points of seed buds minimally and fully. According to the technical key point, the monocotyledon transgenic method comprises the following steps of: germinating seeds for 1 to 2 days, and when buds extend to be 0.2 to 2 centimeters long, removing coleoptile to expose the growing points; pricking and brushing the growing points by using a minimally-invasive brush of which the diameter of a single brush hair is 4 to 20 micrometers, the exposed length is 0.5 to 3 millimeters and the number of the brush hairs is 100 to 5,000 and which is dipped with agrobacterium mediated conversion liquid to perform full minimally-invasive conversion; after performing co-culture, developing further to form seedlings, promoting the development of spikes and grains, and harvesting in a plant division mode; and performing identification on T1 generation. The monocotyledon transgenic method has the advantages that tissue culture, the limit of genotypes a