Method for preparing high-activity lactic acid bacteria by using granulation lyophilization

The invention relates to a method for preparing high-activity lactic acid bacteria by using granulation lyophilization. The method belongs to the technical field of lactobacillus preparation. The method is characterized by comprising the steps: adding protective agents formed by proportionally mixin...

Full description

Saved in:
Bibliographic Details
Main Authors GUAN QIANQIAN, SONG SUHUA, SHUAI GAOPING, XIE MINGYONG, XIONG TAO
Format Patent
LanguageChinese
English
Published 16.01.2013
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
Online AccessGet full text

Cover

More Information
Summary:The invention relates to a method for preparing high-activity lactic acid bacteria by using granulation lyophilization. The method belongs to the technical field of lactobacillus preparation. The method is characterized by comprising the steps: adding protective agents formed by proportionally mixing 5% by mass percent of trehalose solutions and 10% by mass percent of skimmed milk solutions in the prepared lactobacillus bacterial mud, and the volume percent of the zymotic fluid of the protective agents occupies 10%; atomizing the lactobacillus bacteria suspension which is mixed adequately to form drops; spraying into a collecting container of a refrigerating chamber of 60 DEG C below zero to 100 DEG C below zero for refrigerating granulation; continually blowing liquid nitrogen of 196 DEG C below zero into the refrigerating chamber from the side surface of the refrigerating chamber; and fast collecting lactobacillus grains which are refrigerated fast after a spraying process for vacuum refrigerating and dryin
Bibliography:Application Number: CN20121342868