Separation and application of Nectria fungi for promoting growth of Cymbidium wenshanense tissue culture plants

• Main Authors: CHEN SISI, HONG QUNYAN, DONG WENHAN, YANG GENHUA, LI ZHILIN, DUAN CHUNFANG
• Format: Patent
• Language: Chinese; English
• Published: 16.01.2013
• Subjects: AGRICULTURE; ANIMAL HUSBANDRY; BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; FISHING; FORESTRY; HUMAN NECESSITIES; HUNTING; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; NEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; TRAPPING; VINEGAR; WINE

The invention relates to separation and an application of Nectria fungi for promoting growth of Cymbidium wenshanense tissue culture plants and belongs to the field of plant protection and biological technology. The produced strain is Neonectria macrodidyma spp. strain, and its collection number is CGMCC No.3929. The strain provided by the invention has an obvious characteristic of promoting the growth of orchid tissue culture plants. According to the invention, symbiotic fungi obtained by separation is inoculated to Cymbidium wenshanense tissue culture seedling by two methods; the separated strain undergoes preliminary identification through morphological features; and combining molecular biology such as ITS amplification, cloning and sequencing methods, etc., the screened symbiotic fungi which can promote the growth of Cymbidium wenshanense tissue culture plants is identified as the Neonectria macrodidyma spp. fungi. The invention can solve the problem of low survival rate of transplanting orchid tissue cul