Method for detecting affinity indicators of reversible binding of insulin and serum protein and application thereof

• Main Authors: LUAN LIJUAN, CHEN SIFENG, WANG LI, HOU YANQIANG, LU CHAO, ZHANG XUEQING, MENG DAN, LI XIAOBO, XIANG MENG, ZHAO FENGDI
• Format: Patent
• Language: Chinese; English
• Published: 02.01.2013
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention belongs to the technical field of biology and relates to detection of insulin/serum protein binding indictors and particularly relates to a method for detecting affinity indicators of reversible binding of insulin and serum protein and application thereof. The method includes detection of protein-binding insulin, free insulin and bound/free insulin ratio and determination of protein-capturing ability of insulin. Based on the in vitro biochemical study, the study in cultured cell model, the study of patient serum marker detection, the study on drugs and serum components affecting the affinity of reversible binding of insulin and serum protein, the study on impact of change of affinity of binding of insulin and serum protein on blood sugar level in healthy animal and diabetes animal models, it is proven that the detected indicators obtained by the inventive method can further provide experimental data and theoretical basis for prewarning, early diagnosis, prognosis, drug screening and prevention a