Method and special primers for fast identifying phytophthora capsici leonian resistance to pyrimorph

• Main Authors: LIU PENGFEI, CHEN LEI, LI JIANQIANG, LIU XILI, PANG ZHILI, TAN ZHAOHAI, SHAO JINGPENG
• Format: Patent
• Language: Chinese; English
• Published: 02.01.2013
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention relates to identification of nucleotide point mutation of a phytophthora capsici leonian-cellulose synthase-related gene CesA3 and a use of the phytophthora capsici leonian-cellulose synthase-related gene CesA3 in detection of resistance to pyrimorph as a fungicide, and discloses a molecular detection method and special primers for identifying phytophthora capsici leonian gene CesA3 3365-site nucleotide mutation-caused resistance to pyrimorph. The pair of special primers is composed of DNA molecules shown in the formulas of SEQ ID NO: 2 and SEQ ID NO: 3. The molecular diagnosis method for detecting phytophthora capsici leonian resistance to pyrimorph as a fungicide has high sensitivity, good stability and a short detection period, can be used for high-flux detection of generation and development of field phytophthora capsici leonian resistance to pyrimorph as a fungicide, realizes early warning of diseases with resistance and has an important meaning for timely and effective control of developme