Rapid diagnosis method for plesimonas shigelloides

The invention provides a method for detecting plesimonas shigelloides. The method comprises the following steps of: adopting a loop-mediated isothermal amplification technology; using a hug A gene as a target gene; putting designed three pairs of DNA (Deoxyribonucleic Acid) primers and a DNA templat...

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Bibliographic Details
Main Authors MENG SHUANG, XU JIANGUO, YE CHANGYUN
Format Patent
LanguageChinese
English
Published 19.12.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a method for detecting plesimonas shigelloides. The method comprises the following steps of: adopting a loop-mediated isothermal amplification technology; using a hug A gene as a target gene; putting designed three pairs of DNA (Deoxyribonucleic Acid) primers and a DNA template to be detected to an amplification buffer solution including MgSO4 and glycine betaine; amplifying in a constant-temperature environment of 65 DEG C, and terminating the reaction in a constant-temperature environment of 80 DEG C; and detecting the amplified product. The method disclosed by the invention is simple and easy to operate, and has good specificity, sensitivity and repeatability.
Bibliography:Application Number: CN20121262462