Construction method of etr1 gene containing binary expression vector and application thereof

The invention discloses a construction method of an etr1 gene containing binary expression vector and an application thereof. The construction method comprises the following steps of: amplifying an Arabidopis thaliana mutant etr1 gene and cloning it onto an expression vector pEASY-T1 to obtain a pEA...

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Bibliographic Details
Main Authors BEI XUEJUN, ZHANG YIXIU, ZHANG LINGYUN, WANG JI, ZHONG GUANGYAN
Format Patent
LanguageChinese
English
Published 19.12.2012
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TRAPPING
VINEGAR
WINE
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Summary:The invention discloses a construction method of an etr1 gene containing binary expression vector and an application thereof. The construction method comprises the following steps of: amplifying an Arabidopis thaliana mutant etr1 gene and cloning it onto an expression vector pEASY-T1 to obtain a pEASY-T1-etr1 plasmid, carrying out enzyme digestion on AscI and BamHI restrictive endonuclease sites of the plasmid by a double enzyme digestion system to obtain an etr1 gene fragment, carrying out double enzyme digestion on a pFGC5941 plasmid by the same double enzyme digestion system to obtain a backbone fragment, linking the etr1 gene fragment and the backbone fragment by the use of T4DNA ligase to form a circular plasmid. Therefore, the pFGC5941-etr1 binary expression system is obtained. If the system is applied in fresh cut flowers, the service life of the fresh cut flowers can be prolonged for 5-10 days.
Bibliography:Application Number: CN20111162246