Monolithic column electroelution apparatus and method thereof

The invention relates to a monolithic column electroelution apparatus and a method thereof. The monolithic column electroelution apparatus comprises an electrophoresis tank with electrodes, a gel plate containing a target protein zone or a nucleic acid zone, slots, a dialysis tube, and a frame, wher...

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Bibliographic Details
Main Authors CAO CHENGXI, LI GUOQING, LIU XIAOPING, FAN LIUYIN, SHAO JING, GUO CHENGANG
Format Patent
LanguageChinese
English
Published 12.12.2012
Subjects
CHEMISTRY
DERIVATIVES THEREOF
METALLURGY
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PEPTIDES
SUGARS
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Summary:The invention relates to a monolithic column electroelution apparatus and a method thereof. The monolithic column electroelution apparatus comprises an electrophoresis tank with electrodes, a gel plate containing a target protein zone or a nucleic acid zone, slots, a dialysis tube, and a frame, wherein the gel plate is placed in the slot, the dialysis tube is sleeved outside the slot, both ends of the dialysis tube are folded upward, the slot is placed inside the frame, and long axis of the slot is parallel to the electrodes on the outer sides of the frame. According to the present invention, the difficult problem of sample recovery is overcome by assembling simple materials in the experiment, such that characteristics of high sample recovery rate and short elution time are provided, elution of a plurality of samples can be concurrently performed, operation is simple, and the method of the present invention can be adopted as an ideal elution and pre-enrichment method.
Bibliography:Application Number: CN20121287132