Multiplex polymerase chain reaction (PCR) method and kit for detecting human RhD blood type genotypes

• Main Authors: WANG MANNI, WU DAZHOU, XU HUA, YE SHIHUI, DUAN YONG, ZUO QINQIN
• Format: Patent
• Language: Chinese; English
• Published: 21.11.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to a multiplex polymerase chain reaction (PCR) method capable of detecting human RhD blood type genotypes and an in vitro diagnostic kit prepared by applying the method and capable of detecting the human RhD blood type genotypes comprising D/D, D/d, DEL, DEL/d and d/d genotype. According to the method, variation of specific sequence loci of the different genotypes of a human RhD blood type is utilized to design a plurality of pairs of primers to perform a PCR. Accordingly, the multiplex PCR method and the in vitro diagnostic kit can be regarded to be on the basis of a PCR-SSP (sequence specific primer) principle. By designing the sequence specific primers according to different loci and by optimizing mixing proportion, reaction buffer solution ingredients and PCR reaction conditions of the sequence specific primers, the aim of accurately detecting the human RhD blood type genotypes is achieved. In an embodiment, the multiplex PCR method for detecting the human RhD blood type genotypes is