Detection method, probe set, and diagnostic kit for predicting postoperation recurrence-free survival of colorectal cancer via gene counting method

• Main Authors: CAI SANJUN, XU YE, WANG HAIFENG, HUANG WEI, NIU ZHENMIN, WANG ZHIMIN, PENG JUNJIE
• Format: Patent
• Language: Chinese; English
• Published: 07.11.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The present invention discloses a detection method for predicting the postoperation recurrence-free survival of colorectal cancers via a gene counting method, including the steps of: collecting cancerous tissue specimens excised in an operation of a colorectal cancer patient, extracting RNA samples, purifying the RNA samples, detecting the expressions of four specific probes, and calculating the number of a high expression probe to evaluate the patient's prognosis. The invention also discloses a probe set composed by the four probes, and a diagnostic kit containing the probe set. The detection method of the invention helps to improve the survival rate of the colorectal cancer patients. The risk degree of postoperative recurrence and metastasis occurrence of the patients can be quickly determined through the detection and the joint analysis of the four specific probes after that the colorectal cancer patients receive radical operations, so the detection method enables adjuvant treatments for the patients with