Salmonella typhimurium X3306lux and applications of salmonella typhimurium X3306lux in living body imaging

• Main Authors: HUANG RUI, CHU YUANYUAN, YE YING, LI YUANYUAN
• Format: Patent
• Language: Chinese; English
• Published: 31.10.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; PROCESSES USING MICROORGANISMS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a salmonella typhimurium X3306lux and applications of salmonella typhimurium X3306lux in living body imaging. The bacterial strain is preserved in depositary authority designated by the state intellectual property office, the preservation data is 13 March, 2012, the depositary authority is China general microbiological culture collection center, and the preservation number is CGMCC No.5895. The salmonella typhimurium X3306lux is a virulent strain carrying virulent plasmids pStSR100, can be taken as a standard strain, and is applied to the research of the pathogenic mechanism of salmonella typhimurium. X3306lux is the bacterial strain with stable bioluminescence property, which is constructed based on X3306. By the site-specific recombination, a housekeeping gene E.colifrr promoter and a Lux operon are inserted into chromosome of X3306 for stable expression (meanwhile, the insertion site does not damage the gene functions of a genome, and the bacteria can not suffer from the adverse inf