Kit for in-vitro separation purification of karyocytes and removal of erythrocytes and use method of the kit

• Main Authors: CHI MINGGUO, CAI YUNSONG
• Format: Patent
• Language: Chinese; English
• Published: 24.10.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS THEREOF; CULTURE MEDIA; ENZYMOLOGY; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a kit for in-vitro separation purification of karyocytes and removal of erythrocytes. The kit comprises a precipitation solution, a purification solution, a suspension solution and an erythrocyte lysis solution, wherein the precipitation solution, the purification solution, the suspension solution and the erythrocyte lysis solution are separatedly packaged. The precipitation solution is composed of normal saline and hydroxyethyl starch dissolved in the normal saline and has a weight volume concentration of 6%. The purification solution is a mixture of polysucrose and sodium diatrizoate. The suspension solution is normal saline. The erythrocyte lysis solution comprises a stock solution 1 and a stock solution 2, wherein the stock solution 1 and the stock solution 2 are separatedly packaged; the stock solution 1 comprises 10g/L of glucose, 0.6g/L of potassium dihydrogen phosphate, 3.58g/L of Na2HPO4.7H2O and 0.1g/L of a phenol red solution; and the stock solution 2 comprises 1.86g/L of Ca