Preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof

The invention provides a preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof. The method comprises the following steps of: (1) culturing cells for preparing vaccine by a microcarrier, and selecting a bioreac...

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Main Authors ZHOU ZHI, CHEN RUIAI, LIN QIPING, XU JIAHUA, YU XIULING, ZHAI XINYAN, QU PING, LIAN BINGZHOU, TIAN KEGONG, DENG XIAOXIONG
Format Patent
LanguageChinese
English
Published 17.10.2012
Subjects
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
HUMAN NECESSITIES
HYGIENE
MEDICAL OR VETERINARY SCIENCE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS
SPIRITS
VINEGAR
WINE
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Summary:The invention provides a preparation method of porcine reproductive and respiratory syndrome inactivated vaccine (NVDC-JXA1 strain) by bioreactor and application thereof. The method comprises the following steps of: (1) culturing cells for preparing vaccine by a microcarrier, and selecting a bioreactor; (2) propagating and harvesting venom for preparing the vaccine; (3) preparing the vaccine; and (4) inspecting a finished product. The vaccine produced by the method has the advantages of low pollution probability, high virus titer (titer can be increased by 2 to 5 times), small difference among batches, stable product quality and small toxic reaction, so that the vaccine quality can be comprehensively improved.
Bibliography:Application Number: CN201210231129