Method for determining total content of glucosinolates in plants

• Main Authors: GUO QIANGHUI, GU ZHENXIN, WANG ZHIYING, GU YINGJUAN, HAN YONGBIN
• Format: Patent
• Language: Chinese; English
• Published: 26.09.2012
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for determining total content of glucosinolates in plants, belonging to the field of food biotechnologies. The method is characterized in that the plants are taken as testing materials, raw materials are crushed and then homogenized by using an acetate buffer, a homogenate is subjected to high-temperature water bath enzyme deactivation, then mixed liquor of polyvinylpyrrolidone and lead acetate/barium acetate and a saturated sodium sulfate solution are sequentially added, and a glucosinolate extracting solution is produced through centrifuging after carrying out shaking to mix homogeneously; the glucosinolate extracting solution is subjected to alkaline degradation and neutralized by concentrated hydrochloric acid of 37%, a neutralized solution reacts with a potassium ferricyanide solution, and then, the decrement of a light absorption value of potassium ferricyanide in the position of 420 nm is determined by using a spectrophotometer; and a standard curve is made by taking s