Method for preparing biodiesel by directly using cellulose through mixed fermentation

• Main Authors: FEI JUANJUAN, GU XUDING, JI GENGSHENG, LI QIANG, TANG YUBIN
• Format: Patent
• Language: English
• Published: 04.03.2015
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; SPIRITS; VINEGAR; WINE

The invention discloses a method for preparing biodiesel by directly using cellulose through mixed fermentation. The mixed fermentation is performed by using a plant fiber degrading strain and a grease producing strain, and ethanol is produced by directly using natural plant fiber such as straws. The method comprises the following steps of: designing a culture medium for the mixed fermentation by optimization methods such as a response surface method, and optimizing to obtain the fermentation conditions such as the optimum temperature of mixed fermentation, optimum mixing proportion of strains and optimum mixing time of the strains by utilizing the methods such as orthogonal test and response surface methodology; according to the optimized fermentation conditions, by using the plant fiber as a carbon source, degrading the cellulose into fermentable sugar by using a cellulase producing strain, and adding grease-producing microorganisms into the culture system for the mixed fermentation; and by using the sugar which is produced by the constant enzymolysis of the plant fiber as the carbon source, fermenting to produce grease by using the grease producing strain, and performing methyl esterification reaction of the microbial grease to obtain the biodiesel.