Application of small activating RNA (Ribonucleic Acid) of INTS6 (Homo Sapiens Integrator Complex Subunit 6) gene to preparation of prostate cancer fighting medicament

• Main Authors: HU ZHENGHUI, LIN YIWEI, MAO YEQING, XIE LIPING, JIN YONGFENG, CHEN HONG, YANG KAI, QIN JIE, MAO QIQI, ZHENG XIANGYI
• Format: Patent
• Language: Chinese; English
• Published: 12.09.2012
• Subjects: HUMAN NECESSITIES; HYGIENE; MEDICAL OR VETERINARY SCIENCE; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS

The invention provides application of small RNA (Ribonucleic Acid) of a targeted up-regulation INTS6 (Homo Sapiens Integrator Complex Subunit 6) gene to preparation of a hormone refractory prostate cancer fighting medicament. A nucleotide sequence of the small RNA consists of four pieces of positive-sense strands and anti-sense strands of 21 nucleotides; two nucleotides, which are generally dTdT, are overhung at the end 3' of each strand; and the middle 19 nucleotides are paired. According to the application, the expression of INTS6 gene in a hormone refractory prostate cancer cell is induced by the small double-stranded RNA of a specific sequence, so that the proliferation capacity and the movement capacity of tumor cells are inhibited, and the aim of inhibiting tumor growth and development is fulfilled. The small RNA is easy to operate, low in cost and less in using amount, and a good activation effect can be achieved with 20 nM transfection concentration; and the activation effect is clear, and the target