Method for detecting musk xylene in tobacco additive

• Main Authors: MIAO MINGMING, WEI YULING, CHEN JIANHUA, GENG YONGQIN, YANG YEKUN, MIAO ENMING, ZHOU LAN, LI XUEMEI, XU JICANG
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2012
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for detecting musk xylene in a tobacco additive and belongs to the technical field of detection of tobacco additives. The method comprises the following steps of: weighing 1.0g of tobacco additive and putting into a 50mL conical flask with a plug, adding 10mL of ultrapure water of which electrical resistivity is 18.2 M Omega and dispersing a sample, adding 0.3mL of D15-musk xylene interior label solution with the concentration of 1mu g/mL, and 10mL of cyclohexane, oscillating and extracting at the temperature of 25 DEG C for 10 minutes, centrifuging a mixed solution at rotation speed of 4,000r/min by a centrifugal machine for 10 minutes, extracting an upper-layer organic phase, adding 10mL of chromatographically pure cyclohexane into a water phase of which the upper-layer organic phase is extracted, performing secondary extraction under the same operation conditions, centrifuging, and extracting an upper-layer organic phase; and mixing the organic phases extracted twice, dryi