Method for sheeting peanut root tip cell chromosome in mitosis metaphase

• Main Authors: HUANG BINGYAN, DONG WENZHAO, DU PEI, TANG FENGSHOU, ZHANG XINYOU, YI MINGLIN
• Format: Patent
• Language: Chinese; English
• Published: 18.07.2012
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention relates to a method for sheeting peanut root tip cell chromosome in the mitosis metaphase, which comprises the following steps that: a peanut root tip is selected, and paradichlorobenzene is utilized for soaking pretreatment; the root tip is put into carnoy stationary liquid for room temperature treatment; acetic acid is used for soaking, the root tip of a meristematic zone is cut and put on a glass slide, the center of a cover glass is tapped to disperse the cells, a cell wall is heated by fire for dissociating and softening, and a filter paper is used for removing the excess acetic acid; the cell which is in the mitosis metaphase and has smoothly dispersed chromosome is sheeted and refrigerated, the cover glass is removed, and the glass slide is dehydrated; and stain zoning or fluorescence in-situ hybridization are carried out. The obtained sheet has leveled cells and dispersed chromosome, and more cells are in the metaphase; and the acetic acid medium is used for direct compression, the reage