Method for in-vitro induction of new sterile roots from roots of forest stand of rubber plant

The invention relates to a method for in-vitro induction of new sterile roots from roots of a forest stand of a rubber plant. The method comprises the following steps of: selecting the small and tender roots in the diameter of 1.2-1.5mm of the forest stand of the rubber plant, cutting into root segm...

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Bibliographic Details
Main Authors HUANG HUASUN, LI ZHE, HUA YUWEI, HUANG TIANDAI, ZHOU QUANNAN, CAI HAIBIN, DAI XUEMEI, SUN AIHUA
Format Patent
LanguageChinese
English
Published 18.07.2012
Subjects
AGRICULTURE
ANIMAL HUSBANDRY
FISHING
FORESTRY
HUMAN NECESSITIES
HUNTING
NEW PLANTS OR PROCESSES FOR OBTAINING THEM
PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
TRAPPING
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Summary:The invention relates to a method for in-vitro induction of new sterile roots from roots of a forest stand of a rubber plant. The method comprises the following steps of: selecting the small and tender roots in the diameter of 1.2-1.5mm of the forest stand of the rubber plant, cutting into root segments in the length of 8-10mm as explant materials, inoculating into a sterile root induction culture medium for dark culture for 25-40 days, and directly inducing the new roots, wherein the culture temperature is 24 DEG C-30 DEG C. The sterile root induction culture medium comprises 400-450mg/L of CaCl2, 500-520mg/L of KH2PO4, 40-50mg/L of MnSO4.4H2O and 600-620mg/L of MgSO4.7H2O; and 0.5-2.5mg/L of 6-BA (6-benzyl aminopurine), 0.8-1.0mg/L of KT (kinetin), 1.0-3.0mg/L of NAA (1-naphthaleneacetic acid), 5.0-5.5mg/L of 2,4-D (2,4-dichlorophenoxyacetic acid), 2-3g/L of Phytagel, 50-90g/L of sucrose and 40-90ml/L of coconut water are further added. According to the method disclosed by the invention, the roots of the fo
Bibliography:Application Number: CN2012146847