Method for distinguishing European or Asian gypsy moth

• Main Authors: AN YULIN, ZHU HONGBIN, ZHENG SIZHU, YANG XIAOJUN, WANG XIAOLIN, XU MEI, QIAN LU
• Format: Patent
• Language: Chinese; English
• Published: 04.07.2012
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; SPIRITS; VINEGAR; WINE

The invention relates to a method for distinguishing an European or Asian gypsy moth, which comprises the following steps: designing a primer pair and a probe sequence; by using the total DNA (deoxyribonucleic acid) of a sample as a DNA template, upon the completion of real-time fluorescent quantitative PCR (polymerase chain reaction), when an ABI7500 real-time fluorescent quantitative PCR instrument shows that the sample detection result is an AlleleY value, indicating that the basic group at the 406 site of the sample is G, i.e. the sample is an Asian gypsy moth; and when the sample detection result is shown as an AlleleX value, indicating that the basic group at the 406 site of the sample is A, i.e. the sample is an European gypsy moth. The method realizes fast detection, can accurately identify European and Asian gypsy moths, and is applicable to the detection of wood imports at the port.