Method for separating and purifying fructooligosaccharides

• Main Authors: XIAO ANFENG, CAI HUINONG, DU XIPING, LI LIJUN, NI HUI, HUANG GAOLING, YANG YUANFAN
• Format: Patent
• Language: Chinese; English
• Published: 02.05.2012
• Subjects: ACYCLIC OR CARBOCYCLIC COMPOUNDS; BEER; BIOCHEMISTRY; CHEMISTRY; DERIVATIVES THEREOF; ENZYMOLOGY; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE; METALLURGY; MICROBIOLOGY; MUTATION OR GENETIC ENGINEERING; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC CHEMISTRY; PROCESSES USING MICROORGANISMS; SPIRITS; SUGARS; VINEGAR; WINE

The invention discloses a method for separating and purifying fructooligosaccharides, wherein separating and purifying steps include: fermenting cane sugar through Phaffia rhodozyma JMU-MVP14 to obtain a fermentation fluid containing the fructooligosaccharides and astaxanthin; centrifugally separating the fermentation fluid through a centrifuge, extracting the lower-layer astaxanthin, heating the supernatant fluid through a boiling water bath for enzyme inactivation and then purifying the supernatant fluid through a microfiltration membrane, wherein the obtained filtrate flows through a chromatographic column filled with an absorption medium to be absorbed; next, eluting the absorption medium using ethanol solution; and evaporating and concentrating the eluate to obtain the fructooligosaccharides. The method is capable of simultaneously separating and purifying the fructooligosaccharides and the astaxanthin in the same batch of fermentation process; the separation operation is low in cost and simple; the obta