Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof

• Main Authors: CHENG ZHI, GU BIAO, YANG ZIJIAN, WU TAIHU, CUI XIUMEI, CHEN FENG, DU YAOHUA
• Format: Patent
• Language: Chinese; English
• Published: 16.11.2011
• Subjects: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES; MEASURING; PHYSICS; TESTING

The invention discloses an automatic bacterial sorting and labeling method based on an immune method. The method comprises the following steps: 1) coating antibody molecules through magnetic beads; 2) coating the antibody molecules through a fluorescent material; 3) adding a sample solution; 4) conjugating the immunomagnetic beads and target bacterial; 5) carrying out magnetic separation; 6) carrying out washing; 7) labeling the target bacterial through the fluorescent material. The invention further discloses an apparatus for realizing the method. According to the present invention, simultaneous sorting and labeling of a plurality of the target bacterial can be realized so as to improve treatment efficiency; compared to the manual sorting and labeling method, liquid flow in the pipe is pushed through accurate actions of a injector pump provided by the apparatus, such that accurate control for the liquid flow can be realized, error due to a manual operation is avoided, the method has high repeatability.