Automatic bacterial sorting and labeling method based on immune method, and apparatus thereof
The invention discloses an automatic bacterial sorting and labeling method based on an immune method. The method comprises the following steps: 1) coating antibody molecules through magnetic beads; 2) coating the antibody molecules through a fluorescent material; 3) adding a sample solution; 4) conj...
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Main Authors | , , , , , , |
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Format | Patent |
Language | Chinese English |
Published |
16.11.2011
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Subjects | |
Online Access | Get full text |
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Summary: | The invention discloses an automatic bacterial sorting and labeling method based on an immune method. The method comprises the following steps: 1) coating antibody molecules through magnetic beads; 2) coating the antibody molecules through a fluorescent material; 3) adding a sample solution; 4) conjugating the immunomagnetic beads and target bacterial; 5) carrying out magnetic separation; 6) carrying out washing; 7) labeling the target bacterial through the fluorescent material. The invention further discloses an apparatus for realizing the method. According to the present invention, simultaneous sorting and labeling of a plurality of the target bacterial can be realized so as to improve treatment efficiency; compared to the manual sorting and labeling method, liquid flow in the pipe is pushed through accurate actions of a injector pump provided by the apparatus, such that accurate control for the liquid flow can be realized, error due to a manual operation is avoided, the method has high repeatability. |
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Bibliography: | Application Number: CN2011189247 |