Specific primer and liquid-phase chip for polymorphism detection in chromosome 6q23 section

• Main Authors: XU JIASEN, GUO YUANJIE, TAN XIAOXIA
• Format: Patent
• Language: Chinese; English
• Published: 26.10.2011
• Subjects: BEER; BIOCHEMISTRY; CHEMISTRY; COMPOSITIONS OR TEST PAPERS THEREFOR; COMPOSITIONS THEREOF; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES; CULTURE MEDIA; ENZYMOLOGY; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS; METALLURGY; MICROBIOLOGY; MICROORGANISMS OR ENZYMES; MUTATION OR GENETIC ENGINEERING; PROCESSES OF PREPARING SUCH COMPOSITIONS; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; SPIRITS; VINEGAR; WINE

The invention discloses a specific primer and a liquid-phase chip for polymorphism detection in a chromosome 6q23 section. The liquid-phase chip mainly comprises a specific primer, microspheres and amplified primers, wherein the specific primer comprises a tag sequence of a 5' terminal and a specific primer sequence of a 3' terminal for target gene polymorphism sites, wherein the specific primer is more than one pair of SEQ ID NO.15 and SEQ ID NO.16 for G107A, SEQ ID NO.17 and SEQ ID NO.18 for G301A, SEQ ID NO.19 and SEQ ID NO.20 for G103A, SEQ ID NO.21 and SEQ ID NO.22 for G219T, SEQ ID NO.23 and SEQ ID NO.24 for T74C, SEQ ID NO.25 and SEQ ID NO.26 for T163C, and SEQ ID NO.27 and SEQ ID NO.28 for T135C; and the tag sequence is a sequence of SEQ ID NO.1 to SEQ ID NO.14. The prepared liquid-phase chip for polymorphism detection has a good signal-noise ratio, and the designed probe and an anti-tag sequence do not have cross reaction basically.